The rise from a 3 to 7 progesterone level is believed to . Variability in the timing of ovulation, length of oestrus and multiple breeding dates can make it difficult to predict an exact due date for a litter. The Guide Dogs Breeding Centre takes blood samples from their bitches as part of their reproductive management, so it was a valuable . Diestrus: Normally begins 7 to 9 days post the actual LH surge . For fresh chilled and frozen semen, we usually want the progesterone between 15 and 22. When fertility is optimal, progesterone values might be around 10, 30, sometimes even greater than 60ng/mL. Most mammals ovulate when the estrogen level in the blood is increasing. Progesterone is the most important hormone in female dog reproduction. Progesterone testing is usually done every other day and, in the early days of the cycle, may be done every 3 to 4 days. . Progesterone, another important reproductive hormone in the bitch, is present in low levels before the LH surge and then begins to rise around the time of the LH surge. Canine Birth and C-Section Timing Keep the danger at bay. Assessment of different methods of measuring progesterone. eminent to prevent delivery of premature puppies. This baseline progesterone is probably of adrenal origin. Understanding how the hormone levels change can help in determining the best time to breed and when to anticipate whelping. We cannot say that the bitch should be bred when she is at [any number] ng/mL of progesterone. Progesterone levels of 35.0 - 40.0 ng/ml on the miniVidas results; LH testing is rarely used today, however, it was most used when planning a frozen semen breeding or when breeding a bitch that has a history of difficulty of conceiving. Whether you have a small or large breed, your bitch should ovulate at around the same progesterone level as any other dog. August 17th 1.4. Ovulation happens when progesterone blood levels reach 5 or 6ng/mL. Progesterone timing at the end of pregnancy. The progesterone levels, like the speed of the car, is determined by the individual. LH causes the luteinization of the ovarian follicles after the eggs are released. A progesterone below 3 ng/dl indicates a c-section is safe, as long as the dog does not have luteal failure. Dr. Loretta. August 22nd 5.1. This represents a huge saving in lost time of empty bitches and generally maximising litter size as well. If you get readings that do not make sense, then re-test. The ovulation date can be as early as day 7 and as late as day 27 of the estrous cycle, emphasizing the importance of the blood test. The average bitch will cycle approximately every 6 months and an individual bitch will tend to conform to her own unique repeating pattern throughout her fertile years. This baseline progesterone is probably of adrenal origin. The overall investment including also the cost of a quality bitch will be a lot of money. Breeding with fresh semen, by either natural breeding or vaginal AI with fresh semen, can be done on the day of ovulation, when the progesterone level reaches 5 ng/ml. The first vaginal artificial insemination should be performed 48 hours after the progesterone reaches 5 ng/ml. progesterone level. Progesterone levels and luteinizing hormone (LH) levels are the best indicators of when ovulation will take place and when is the best time to breed. Important note: The above are guidelines, and individual dogs may vary. When fertility is optimal, progesterone values might be around 10, 30, sometimes even greater than 60ng/mL. LH tests must be done daily and the blood must be drawn at approximately the same time each day. We are proud to be a Brucellosis-free hospital; We require a Brucellosis negative certificate within the last 6 months for all breeding dogs; Same day results along with a certificate of the negative test status; Breeding Timing with Progesterone Levels Dogs, however, ovulate when the estrogen level is already declining and the progesterone level is increasing. The most fertile time to breed is 2 to 4 days after ovulation (C4). 1. These areas on the ovaries are where progesterone is produced in levels high enough to maintain Call the stud owner as soon as the bitch is showing signs . It is recognized that in the dog up to 75% of failures to conceive can be attributed to incorrect timing of breeding. In my last breeding, the dog had three tests with progesterone readings of 3+ and then a reading of 5. Serial progesterone measurements can be collected by sampling every 1 to 4 days - increasing in frequency as the sharp rise in progesterone approaches. These areas on the ovaries are where progesterone is produced in levels high enough to maintain Typically we like to pull progesterone levels on Day 5 of the bitches heat cycle. Using our lab ovulation occurs at about 3.8-5.0 ng/ml (11.7 nmol/L) of progesterone (including the day of ovulation and the 24 hours it takes to ovulate) and the best days to breed occur at about 17.6-24.1 ng/ml (54-74 nmol/L) of progesterone. Progesterone levels are not species specific, which means that the test can be run in almost any veterinary lab, or in even human labs. conducted a comparative study of three different methods of measuring the level of progesterone in blood samples from bitches. 1. Dog breeding is a complicated business. However, most commonly, surgery is required. The dramatic change in Progesterone levels during the breeding period renders it an excellent tool for ovulation timing. This method of deciding when to mate should improve our pregnancy rates from around 70% to 95%. When fertility is optimal, progesterone values might be around 10, 30, sometimes even greater than 60ng/mL. Idexx also recommend when it is you go to mate the dog or if another test is needed (usually 2 days later if the progesterone level was too low for an accurate prediction of ovulation). Progesterone levels are usually less than 1.0 ng/ml before the LH surge, rising to 3 to 5 ng/ml the day following the LH surge. The perfect bitch will ovulate when her progesterone levels reach 5ng/ml. The fertile period of breeding is generally recommended as the 3rd to the 5th days of estrus, or every . Please follow this chart and give to your veterinarian when planning for a breeding. Determine breeding schedule based on desired method of insemination and semen type. 6. As the progesterone level increases, lutenizing hormone (LH) is released from the pituitary gland, triggering the release of eggs from the follicles. This is most effective when you can get progesterone results back the same day the sample is drawn and sent to the lab. Most breeders will opt for a live AI instead of letting the dogs mate in order to avoid injury to the stud dog. Ovulation will occur anytime when progesterone is between 5ng/ml and 10ng/ml. Progesterone Levels Predict Breeding Results. If the reading is 9, you're too late for a natural breeding, if 0.9, you're too early. This value is the same whatever the size of the dog. Progesterone can also be used to estimate the date of whelping. Hormonally it begins on the day of the LH surge and is marked by rising progesterone levels and decreasing estrogen levels. With one easy, on-site test, detect progesterone to accurately predict ovulation and target optimal breeding dates […] Her progesterone levels should also rise from basal levels to over 5 ng/ml. Both vets had recommended breeding on the 24th and 25th based on levels and even on the 23rd when the result of 11 was given they told her to breed the next day. A progesterone below 3 ng/dl indicates a c-section is safe, as long as the dog does not have luteal failure. However, this relationship is not well established and there is considerable variation in serum progesterone concentrations after the initial rise. This type of ovulation timing is used for many reasons…fresh chilled and frozen breeding, infertility evaluations, natural breeding with limited stud access, and breedings with older males or dogs with poor semen quality. Ovulation will occur anytime when progesterone is between 5ng/ml and 10ng/ml. Canine progesterone blood testing takes away all the most important uncertainty out of dog breeding. When the progesterone level is between "4" (55)- "5" (65). The dog progesterone test kit is specific to the state-of-the-art Cube Vet analyzer shown here. Accurate breeding history, especially LH peak, is also very valuable. The duration of pregnancy has always been regarded as 63-65 days from the first mating. Eggs are released (ovulated) 44 hours (2 days) after the LH surge, on average. When testing progesterones at the end of pregnancy, we frequently see . 15-22: Your bitch is ready to . The eggs are viable for about 3 - 4 days after this (5 - 6 days after the LH surge). Number Results Quantitative Number Results in Minutes Breed with confidence. Once a bitch hits 5ng and ovulates, subsequent serum progesterone levels can vary greatly. Thereafter the progesterone is > 5 ng/ml. August 24th 23. Progesterone can also be used to confirm that ovulation did take place- levels over 15.0 ng/ml can be used retrospectively to confirm ovulation, but does not help in future planning of a breeding of that cycle. Serum Progesterone for Estimating Day of Parturition in the Bitch. LH causes the luteinization of the ovarian follicles after the eggs are released. If previous breeding history is unknown, begin progesterone testing 4-6 days after the onset of heat. This is most effective when you can get progesterone results back the same day the sample is drawn and sent to the lab. We would like to minimize misses based on timing. When it has been determined that the dog has ovulated, peak fertility is 48 hours after a progesterone result of 4-10 is noted. However, for the best results, many breeders will mate their dogs five and six days after the bitch's luteinizing hormone surge. Once you start to see the level at: 2.0 to 2.9 ng/ml = breed in 4 to 5 days 3.0 to 3.9 ng/ml = breed in 3 to 4 days 4.0 to 8 .0 ng/ml = breed today or tomorrow - this is where the LH Surge Occurs. Progesterone is at baseline concentrations (<1 ng/ml). 5 part series all about progesterone levels in breeding dogs All about dog breeding www.LoveMyPups.comDon't forget, subscribe, like and make comments, Our st. Broadly: Progesterone is over 6.5 nmol/L (2 ng/ml) at the LH peak: breed 4-6 days later. Progesterone Cycle in the Bitch Is a progesterone level of 15 good? Many breedings are done far too early and result in misses. Pre-Breeding Screening & Testing. Behaviorally it begins the first day the bitch stands. Progesterone timing at the end of pregnancy. We cannot say that the bitch should be bred when she is at [any number] ng/mL of progesterone. Timing of breeding The aim is to identify when the progesterone level reaches 2.5 ng/ml so the mating schedule can be set up, or the veterinarian and owner of the male dog can be notified that they should be prepared to collect and ship a semen sample or to pinpoint the optimum time for breeding Thus, a bitch should begin breeding 2-4 days after the progesterone rises over 2 ng/ml. My name is ***** ***** and I am trying to breed two Labs. As a result, the prime time to breed is 48 to 72 hours post ovulation." Progesterone kits that chart progesterone levels through color changes are available to breeders from veterinarians. Be secure. Monitor your dog's progesterone levels to determine the proper C-section timing. Although the eggs are not mature yet, most fresh semen is viable enough to survive in the bitch's reproductive tract until the eggs are ready. However, at the time of breeding, there is no "ideal" level. In order to interpret results one needs to be aware of the unique pattern of progesterone secretion in the bitch. Progesterone levels. Large and small animal Vet. To time C-section or whelping, test progesterone starting 58 - 60 days after first breeding (average gestation 63 days). Progesterone levels should be tested every two to three days once the dog is five(5) to seven(7) days into heat. If the levels of progesterone are baseline, then the dog should be retested every 3-4 days until a level of progesterone is detected that is consistent with the onset of the LH peak. 22,572 satisfied customers. The breeding protocol should be determined based on the estimated day of ovulation. It improves breeding efficiency and provides confidence for the breeders and veterinarians that the dog and bitch are mating on the correct day. There are some reports that a serum progesterone concentration of 4-10 ng/ml occurs in association with ovulation. The surgery to implant the semen is a August 23rd 11. Progesterone levels are usually less than 1.0 ng/ml before the LH spike, rising to 3 to 4 ng/ml the day following the LH spike. Estrus: Is the period of receptivity. It is important to know that if the rise in progesterone is missed, guesswork is needed to determine the actual day for the breeding. You can usually wait to do the first progesterone number on the 8th day. Determine breeding schedule based on desired method of insemination and semen type. Blood samples will be taken every 2-3 days until the serum progesterone level reaches 4-10, this is the day of ovulation. The ideal progesterone level for mating within 1-3 days is 19-31 nmol/L or 5.97-9.75 ng/mL, readings within this range indicate that ovulation has recently occurred. Progesterone is over 16 nmol/L (5 ng/ml) at ovulation. Progesterone level monitoring is most effective and easy to use. If your female's progesterone reading is 32-64 nmol/L or 10.06-20.13 ng/mL the eggs have matured and mating should occur within 2 days for optimal fertility. Even spayed bitches run basal levels of progesterone. Blood progesterone levels and fetal monitoring (by ultrasound) are most critical. Using our lab o vulation occurs at about 3.8-5.0 ng/ml (11.7 nmol/L) of progesterone (including the day of ovulation and the 24 hours it takes to ovulate) and the best days to breed occur at about 17.6-24.1 ng/ml (54-74 nmol/L) of progesterone. levels preparing the reproductive tract for breeding. Some progesterone levels advance really quick, like the car off the line in a 1/4 mile test and . So you need certain assurance that your bitch will be 100% ready to breed at a specific time. The breeding protocol should be determined based on the estimated day of ovulation. Progesterone is at baseline concentrations (<1 ng/ml). Why Progesterone Testing Is Important for Dog Breeding August 14, 2018 10:58 pm Published by Writer Leave your thoughts. 2.0 to 5.0: Test every day. The breeding protocol should be determined based on the estimated day of ovulation. Progesterone levels are usually less than 1.0 ng/ml before the LH spike, rising to 3 to 4 ng/ml the day following the LH spike. Over 8.0 ng/ml with no white sells - then the bitch has ovulated When you hit the level above 4.0 ng/mL this is day 1 of ovulation. If the level is less than 5ng /ml another value should be checked a few days later. It's also one that requires you to be aware of a variety of details about your dog's health and wellness. . Occasionally, medical intervention will suffice, including calcium or magnesium supplementation. What we are looking for is a progesterone level of 20nmol/l. Progesterone Levels Predict Breeding Results Progesterone levels should be tested every two to three days once the dog is three to five days into heat. This indicates that ovulation is imminent. If a breeder is not in a position to do a complete progesterone level series there is still an added value running a progesterone level when the bitch starts flagging and again on the first breeding day. A drop in progesterone to < 2 ng/mL (from C4 to dark C2 or C1) indicates whelping will occur within 24 - 36 hours. If you get readings that do not make sense, then re-test. Dogs, however, ovulate when the estrogen level is declining and the progesterone level is increasing. Most bitches ovulate between a 3ng and 7ng so watch for a 5.0 as your benchmark. The progesterone test (needle of the speedometer) reflects the amount of progesterone being released. The units they deliver the results in are normally ng/ml (nanogram per millilitre of blood). The reason it is so difficult to time ovulation in the absence of hormone testing is that the stages of the canine estrous cycle vary considerably in length. Continued sampling will confirm ovulation. We usually start natural breeding's the day the progesterone goes over 10. The first vaginal artificial insemination should be performed 48 hours after the progesterone reaches 5 ng/ml. Most veterinarians recommend breeding 2 days after ovulation with fresh and fresh chilled shipped semen and 3 days with frozen semen. As the cycle continues, progesterone levels slowly climb to a level of 5ng, upon which ovulation occurs. You want healthy puppies! At what progesterone level should I breed my dog? dog progesterone level chart dog ovulation cycle. August 21st 4.7. When fertility is optimal, progesterone values might be around 10, 30, sometimes even greater than 60ng/mL. Important note: The above are guidelines, and individual dogs may vary. On a side note-this elevated progesterone level is what causes the symptoms of a false pregnancy when a bitch is not pregnant. What level of progesterone indicates ovulation in dogs? However, at the time of breeding, there is no "ideal" level. . The fertile period of breeding is generally recommended as the 3rd to the 5th days of estrus, or every . The breeding protocol should. Progesterone, another important reproductive hormone in the bitch, is present in low levels before the LH surge and then begins to rise around the time of the LH surge. August 20th 1.9. Here are the progesterone levels. With my dogs the progesterone spike seems to happen 9-11 days following first color, but that varies from dog to dog. Progesterone levels can sometime double in 24 hours so daily testing is imperative. We determine breeding days for natural or transcervical breeding at 3 and 5 days after the . Even spayed bitches run basal levels of progesterone. Larger dogs tend to reach puberty at an older age than smaller dogs. In 2010, Moxon et al. . Breeding the bitch on days 3 and 5 (or 4 and 6) after the LH surge ensures the maximum conception rate and largest litter sizes. When the progesterone level rises to 5 ng/ml, ovulation occurs. This is the cost per test and many breeders will take 2 or even 3 tests over several days until they get the correct date and progesterone levels that signal the dog is ready to breed. After ovulation, progesterone concentrations continue to increase for 2-3 weeks reaching as much as 80 ng/ml, this level is necessary to maintain the pregnancy. Vaginal … read more. Larger dogs tend to reach puberty at an older age than smaller dogs. The dog was bred using side by side. If doing a natural breeding, I generally breed the bitch the day of ovulation or the day after, and then two days later(2-3 days after ovulation). We know the progesterone level at the time of ovulation. Estrogen levels can give us a general idea of when a dog will come into "standing" heat, but are not sufficient to determine when breeding should actually take place for optimal conception rates. A reproductive hormone, progesterone increases in the bloodstream just before ovulation. The Reproductive Dog Breeding Cycle Can Be Tricky! It is easy to test for and can be used to look at ovulation, parturition and other cyclical abnormalities. At what progesterone level does a dog ovulate? Progesterone testing is used to determine when a bitch ovulates and thus when to breed. Interpretation of Serum Progesterone Results for Management of Breeding in Dogs Although the eggs are not mature yet, most fresh semen is viable enough to survive in the bitch's reproductive tract until the eggs are ready. Brucellosis Test. Breeding with fresh semen, by either natural breeding or vaginal AI with fresh semen, can be done on the day of ovulation, when the progesterone level reaches 5 ng/ml. Doctoral Degree. The hormone progesterone (P4) is an androgen produced by the corpus luteum of bitches. Christine Most professional dog breeders use either natural or artificial insemination methods to maximize the success of the breeding. It can vary from one cycle (or heat) every 18 months (this is typical of some of the giant breeds) to one every 4 months. The timing of the breeding, based on progesterone levels (bred 2 to 3 days post-ovulation depending on semen type used), was just right. Oestrogen levels can give us a general idea of when a dog will come into heat, but are not sufficient alone to determine when breeding should actually take place. Progesterone is best used in conjunction with other monitoring techniques, such as observation of clinical signs, vaginal cytology, vaginoscopy, ultrasound, and additional hormonal testing. Your dog could be ready almost any day. When the progesterone level rises to 5 ng/ml, ovulation occurs. Explore further detail here. LIVE BREEDING TIMING Venomline's Mrs. Dolce 24 hours (1 day) after progesterone. In the dog, the progesterone level will remain high for about 60 days whether or not the dog is bred, and whether or not she is pregnant. 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